WebJan 10, 2024 · Fluorescein isothiocyanate (FITC) is an organic fluorescent dye and probably one of the most commonly used in immunofluorescence and flow cytometry. It has an excitation/emission peak at 495/517 nm and can be coupled to distinct antibodies with the help of its reactive isothiocyanate group, which is binding to amino, sulfhydryl, imidazoyl ... WebFITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required for optimal staining …
FITC (Fluorescein 5-isothiocyanate) Fluorescent Dye
WebFluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and … WebFITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu 2+ -sensitive fluorescence dye [1] [2] . In Vitro. Annexin V-FITC/PI is employed to detect chondrocyte apoptosis [1]. The PEI-modified CDs are conjugated with fluorescein isothiocyanate (FITC) to fabricate CDs-FITC composites [2]. the park vista doubletree gatlinburg tn
Our Story - FITC
WebFITC (Fluorescein isothiocyanate) is the form of fluorescein used for conjugation to all JIR antibodies and purified proteins, with the exception of streptavidin. Fluorescein conjugates absorb light maximally at 492 nm and fluoresce maximally at 520 nm. Although less bright than other green-fluorescing dyes, FITC is a widely used fluorophore ... WebDextran B512 preparations of various mol. wts induced polyclonal antibody synthesis in vivo. The efficiency of activation decreased with the mol. wt. Dextran also markedly enhanced the anti-hapten ... Webabove 9. FITC-xtra has spectral properties almost identical FITC. In addition, FITC-xtra give much higher conjugation yield under mild conjugation conditions than FITC. Like 5-FITC, FITC-xtra antibody conjugates have excitation ideally suited to the 488 nm laser line, making them alternatives to the corresponding FITC-labeled antibody conjugates. the parkway 1428 7th st e st paul mn 55106