Panc1 transfection
WebSep 16, 2005 · The pancreatic adenocarcinoma cell lines CAPAN1, CAPAN2, CFPAC1, MiaPaCa2, BxPc3, and Panc1 were obtained from the American Type Culture Collection repository (Manassas, VA). The mouse embryo fibroblast line NIH 3T3 was used as control ( 13 ). Plasmids and Transient Transfection of CAPAN1 WebMay 14, 2013 · The Panc1 clone was established by transfection with a reporter construct containing a promoter sequence of CDH1/E-cadherinin the 5′ upstream region of the ZsGreen1reporter gene and cloning using limiting dilution (see Fig. 1A, and 1B). Each dsRNA was transfected individually into the clone.
Panc1 transfection
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WebTo demonstrate the crucial role of ALK5 in mediating TGF-β1-induced chemokinesis, we knocked down ALK5 by transient transfection of wild-type Panc1 cells and Colo357 cells with ALK5 siRNA. In these cells, the TGF-β1 effect on migration was strongly reduced . To obtain evidence that the upregulation of ALK5 is also responsible for the RAC1B KD ... WebDec 2, 2024 · Transfection was carried out by using Lipotransfectamine 3000. Dual-Luciferase Report Assay (Promega) was performed after transfection for 48 h. Chromatin immunoprecipitation assay Briefly, PANC-1 or BxPC-3 cells were cross-linked with 1% PFA. After sonicating and pre-clearing, cells were incubated with appropriate antibodies.
WebMar 3, 2024 · Adherent PANC1 cells were transfected using Lipofectamine 2000 Reagents (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s recommendation. After 4 h from transfection, cultures were used for western blot, real-time PCR, immunofluorescent experiments, cell survival assay as described above. … WebMar 7, 2024 · PANC1 Transfection Kit (Non-endocrine Pancreatic Cn) $ 396.00 – $ 2,070.00 SKU: PANC1 Product Availability: In Stock (FedEx delivery within 2 business …
WebPANC-1 is a human pancreatic cancer cell line isolated from a pancreatic carcinoma of ductal cell origin. [1] PANC-1 was derived from the tissue of a 56-year-old male. The cells … WebThe present study aimed to investigate the role of JWA gene in the proliferation, apoptosis, invasion and migration of PANC-1 pancreatic cancer cells and the effect on the MAPK …
WebHuman PANC-1 cell line has been used to study the role of the intermediate filament keratin reorganization on the alignment and migration of cancer cells. It has also been used to …
WebMay 31, 2024 · The KRAS oncogene, present in over 90% of pancreatic ductal adenocarcinomas, is most frequently the result of one of three gain-of-function … how to check touch screen laptopWebNational Center for Biotechnology Information how to check total timingWebpanc1 Cells. Organism Type: Human: human pancreatic epithelioid carcinoma cell line. Can be obtained from ATCC: Tissues: Pancreas: Phenotype: Adherent: Primary: no: Application: Protein Expression,Transient Transfection: Every step of the way, a wide range of cell health products. Maintaining healthy cells is the key to experimental success and ... how to check touchpad driver in windows 10WebCommonly known as G418 or G-418, Gibco Geneticin reagent is an aminoglycoside related to gentamicin and is commonly used as a selective agent for eukaryotic cells. As an analog of neomycin sulfate, it interferes with the function of 80S ribosomes and protein synthesis in eukaryotic cells. how to check to validate ptinWebMix RNP complexes with PANC-1 CRISPR transfection reagent. For Cas9 protein with an NLS sequence and attached sgRNA, combine 40 μl serum-free medium, 5.5 μl transfection reagent, and 750 ng of RNP complexes. a. If using a repair template, add 50 ng during this step. The repair template must also be encapsulated to ensure high transfection ... how to check towing capacity for my truckWebFigure Legend Snippet: ( A ) Panc1 and MiaPaCa cells were transfected with scrambled and PD2 RNAi oligos and 48 hours post transfection immunofluorescence analysis was carried out. Confocal images show that there is decreased localization of CHD1 in the nucleus (blue, stained with DAPI) of PD2 KD pancreatic cancer cells (middle panel) compared to the … how to check town council conservancy chargesWebPANC-1 is a pancreatic cancer cell line that is commonly used as a model system for studying pancreatic cancer and evaluating potential therapies. Pancreatic cancer is a type of cancer that arises from the cells of the pancreas, which is an organ located in the abdomen that produces enzymes and hormones that regulate blood sugar levels. how to check touchpad drivers