Pyt313
WebDec 13, 2024 · Mutations to the F. johnsoniae chromosome were made using precise allelic replacement (Zhu et al., 2024).Alleles were cloned into the Bam HI and Sph I restriction sites of the suicide vector pYT313 (Zhu et al., 2024).This vector has two selectable markers, bla (expressed in E. coli) and ermF (expressed in F. johnsoniae), as well as the counter … WebOct 1, 2024 · using pYT313 is identical to the above mentioned rpsL-mediated strategy except that 244. wild type C. hutchinsonii can be used as the parental strain and sucrose …
Pyt313
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WebAug 30, 1995 · With multiple (M) mutagenesis, TaG4GSG~'CTGs to A3C4GSC6GTC8 at the right end (pYT315), transpositions are not detected. Interestingly, if both ends are altered to restore an inverted repeat (pYT313), a low but reproduc- ible level of transposition is detected. How can the double mutant rescue be explained'? WebNov 7, 2024 · Finally, plasmid pYT313 35 was linearized by PCR amplification using FL203 and FL204. The three amplicons were then assembled by Gibson cloning resulting in …
WebFeb 21, 2024 · The first step is to insert two DNA fragments upstream and downstream of porY into pYT313. Specific Content: For the past few weeks, we had conducted plasmid preparation from the Escherichia coli bacterium, otherwise known as E. coli, polymerase chain reaction (PCR), gel electrophoresis, purification, and digestion. WebSUPPORTING INFORMATION. Supplementary Methods2. M1. Construction of the bfpR, hfpR, hfpY and hfpRbfpR strains2. M2. Construction of pGi39 plasmid allowing chromosomal gene integra
WebFlavobacterium columnare is an important bacterial pathogen of freshwater fish that causes high mortality of infected fish and heavy economic losses in aquaculture. The … WebThe fragment was digested using BamHI and PstI and ligated into pYT313 that had been digested with the same enzymes, to generate pFT7. A 2037-bp fragment spanning the final 42 bp of ausR and 1995 bp of downstream sequence …
WebJul 7, 2024 · The rfp18 deletion mutant was constructed using a pYT313-derivative plasmid as described in SI Appendix M3. Proteolytic activity was quantified on azocasein …
WebMap of the sacB-containing suicide vector pYT313 used for constructing chromosomal gene deletions. pYT313 was constructed by introducing sacB and the promoter of F. … nottingham city executive boardWebCells of Flavobacterium johnsoniae move rapidly over surfaces by a process known as gliding motility. Gld proteins are thought to comprise the motor that propels the cell … how to shop for golf clubsWebJan 11, 2024 · The vector pYT313 (ref. 38) was linearized by amplification with primers FL313 and FL314. These three fragments were then assembled by Gibson cloning to … how to shop for gold chainsWebFeb 2, 2024 · These two fragments were then cloned into the Bam HI and Sph I sites of suicide vector pYT313, using Gibson Assembly . Resulting plasmids were moved into F. johnsoniae, via tri-parental mating , and erythromycin (Em, 100 μg/mL) resistant transconjugants were selected. nottingham city fair access protocolWebNational Center for Biotechnology Information how to shop for health insurancenottingham city environmental healthWebCells of Flavobacterium johnsoniae exhibit rapid gliding motility over surfaces. Cell movement is thought to involve motor complexes comprised of Gld proteins that propel … nottingham city facts